391016 Zellkulturtechnik (BPr) (WiSe 2024/2025)

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Dieses Modul vermittelt praktische Kenntnisse, die zur Benutzung von Säugerzellkulturen zur Produktion von pharmakologisch bedeutsamen Wirkstoffen notwendig sind. Gegenstand des Praktikums ist die Planung, Modellierung, Optimierung und experimentellen Durchführung einer FedBatch-Kultivierung rekombinanter tierischer Zellen in Rührkesselbioreaktoren zur Produktion eines Glykoproteins. In der Auswertung sollen u.a. die Unterschiede zwischen dem vom Modell vorhergesagten und dem realen Prozessverhalten erarbeitet werden.
Ausgangspunkt der Modellierung und Optimierung des FedBatch-Prozesses sind reale Fermentationsdaten der im Praktikum verwendeten Zelllinie. Dabei kommt das Simulationsprogramm 'Berkeley Madonna' zum Einsatz.In einem weiteren Experiment lernen die Teilnehmerinnen und Teilnehmer den inclined-settler als Zellrückhaltemethode für kontinuierliche Perfusionskulturen kennen und erarbeiten den Einfluss der wichtigsten Betriebsparameter.

Das Praktikum findet im Laborbereich E0 statt.

Teaching staff

Dates ( Calendar view )

Frequency Weekday Time Format / Place Period  
block n. V.   07.10.2024-31.01.2025

Subject assignments

Module Course Requirements  
39-M-MBT11P Spezialisierung Zellkulturtechnik Praktikum Praktikum Zellkulturtechnik Ungraded examination
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Last update basic details/teaching staff:
Saturday, May 18, 2024 
Last update times:
Saturday, May 18, 2024 
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Saturday, May 18, 2024 
Type(s) / SWS (hours per week per semester)
BPr /
Department
Faculty of Technology
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474279060