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Curriculum Vitae

Thomas R. Huser, Ph.D.
University of Bielefeld, Bielefeld, Germany
Department of Physics, D3, Universitätsstr. 25, 33615 Bielefeld, Germany
Email: thomas.huser@physik.uni-bielefeld.de

University of California, Davis
2700 Stockton Blvd., Suite 1400, Sacramento, CA 95817, USA
Email: trhuser@ucdavis.edu

Professional Preparation:
University of Basel, Switzerland Physics M.A. 1992
University of Basel, Switzerland Physics M.S. 1994
University of Basel, Switzerland Physics Ph.D. 1998
Lawrence Livermore National Laboratory, Livermore, CA Materials Science Postdoc 1998-2000

Positions and Employment :
2000–2002 Staff Physicist, Lawrence Livermore National Laboratory (LLNL), Livermore, CA
2002–2005 Scientific Capability Leader “Nanophotonics,” Chemical Biology and Nuclear Science Division, LLNL
2003–2005 Group Leader “Biophotonics,” Medical Physics and Biophysics Division, LLNL
2004–2005 Deputy Director, Physical Biosciences Institute, LLNL
2005–2011 Chief Scientist, Center for Biophotonics Science and Technology, UC Davis
2005–2011 Associate Professor, Department of Internal Medicine, UC Davis
2006–2011 Co-Director, Translational Working Group, Clinical Translational Science Center, UC Davis
2008–2011 Co-Chair, Designated Emphasis in Biophotonics, University of California, Davis
2011- Professor, Department of Physics, University of Bielefeld, Germany
2011- Adjunct Professor, Department of Internal Medicine, University of California, Davis, USA

Professional Societies and Public Advisory Committees:
2005, 2007 ad hoc Member, Special Emphasis Panel “Nanoscience and Nanotechnology”, NIH
2009 ad hoc Member, Physical Sciences Oncology Centers, NCI-NIH
2008 ad hoc Member, “Career” grants, National Science Foundation
2009 ad hoc Member, BISH Panel, National Science Foundation
1994– German Physical Society
1998– American Physical Society

1998 graduated “summa cum laude”, University of Basel, Switzerland
2002 Norbert J. Kreidl Award of the Glass and Optical Division of the American Ceramic Society
2004 Excellence in Publication Award of the Associate Director, Chemistry and Materials Science, LLNL
2006 “Above and Beyond” Award for undergraduate teaching, Center for Biophotonics
2008-2011 Visiting Professor, University of Tromso, Norway
2009 Guest Editor for Journal of Biophotonics on “Biophotonics in Regenerative Medicine”

Most recent Publications:
V. Mönkemöller, M. Schüttpelz, P. McCourt, K. Sørensen, B. Smedsrød, and T. Huser, Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy, Phys. Chem. Chem. Phys. in press (2014), DOI: 10.1039/C4CP01574F

D. Wolfson, M. Steck, M. Persson, G. McNerney, A. Popovich, M. Goksör, and T. Huser, Rapid 3D fluorescence imaging of individual optically trapped living immune cells, J. Biophoton. in press (2014)

F. Humpert, I. Yahiatène, M. Lummer, M. Sauer, and T. Huser, Quantifying molecular colocalization in live cell fluorescence microscopy, J. Biophoton. in press (2014)

I. Yahiatène, S. Hennig, and T. Huser, Optical fluctuation microscopy based on calculating local entropy values, Chem. Phys. Lett. 587, 1-6 (2013), Invited Frontiers Article, Cover Article

I.W. Schie, L. Nolte, T.L. Pedersen, Z. Smith, J. Wu, I. Yahiatene, J.W. Newman, and T. Huser, "Direct Comparison of Fatty Acid Ratios in Single Cellular Lipid Droplets as determined by Comparative Raman Spectroscopy and Gas Chromatography", Analyst 138(21), 6662-6670 (2013)

A. Avetisyan, J. Jensen, and T. Huser, "Monitoring Trehalose Uptake and Conversion by Single Bacteria using Laser Tweezers Raman Spectroscopy", Anal. Chem. 85(15), 7264-7270 (2013)

O. Ampomah, A. Avetisyan, E. Hansen, J. Svenson, T. Huser, J. Jensen, and T. V. Bhuvaneswari, “The thuEFGKAB Operon of Rhizobia and Agrobacterium tumefaciens Codes for Transport of Trehalose, Maltitol and Isomers of Sucrose and Their Assimilation through the Formation of Their 3-Keto Derivatives", J. Bacteriol. 195(17), 3797-3807 (2013)

I.W. Schie, and T. Huser, “Methods, Analysis, and Applications of Raman Microscopy of Single Cells", Appl. Spectrosc. 67(8), 813-828 (2013), Invited Review Article, Cover Article

L. Jiang, I.W. Schie, J. Qian, S. He, and T. Huser, “Coherent anti-Stokes Emission from Gold Nanorods and Its Potential for Imaging Applications”, ChemPhysChem 14(9), 1951-1955 (2013)

S. Ly, R. Altman, J. Petrlova, Y. Lin, S. Hilt, T. Huser, T.A. Laurence, and J. Voss, “Binding of apolipoprotein E inhibits the oligomer growth of amyloid beta in solution as determined by fluorescence cross correlation spectroscopy”, J. Biol. Chem. 228(12), 11628-11635 (2013)

D.K. Lieu, J.-D. Fu, N. Chiamvimonvat, K.W. Chan Tung, G.P. McNerney, T. Huser, G. Keller, C.-W. Kong, R.A. Li, “Mechanism-based facilitated maturation of human pluripotent stem cell-derived cardiomyocytes”, Circ Arrhythm Electrophysiol 6, 191-201 (2013)

I.W. Schie, and T. Huser, “Label-Free Analysis of Cellular Biochemistry by Raman Spectroscopy and Microscopy”, Compr. Physiol. 3, 941-956 (2013), Invited Review Article

P. Løvhaugen, B.S. Ahluwalia, T.R. Huser, and O.G. Hellesø, “Serial Raman spectroscopy of particles trapped on a waveguide“, Opt. Express 21(3), 2964-2970 (2013)

D. Svistounov, A. Warren, G.P. McNerney, D.M. Owen, D. Zencak, S.N. Zykova, T. Huser, R.J. Quinn, B. Smedsrod, D.G. Le Couteur, V.C. Cogger, “The Relationship Between Fenestrations, Sieve Plates and Rafts in Liver Sinusoidal Endothelial Cells“, PLoS ONE 7(9), e46134.
doi:10.1371/journal.pone.0046134 (2012) - featured as „major advance“ in Hepatology (2013)

T. Gao, J. Petrlova, W. He, T. Huser, W. Kudlicki, J. Voss, and M.A. Coleman, “Characterization of de novo synthesized GPCRs supported in nanolipoprotein discs“, PLoS ONE 7(9), e44911. doi:10.1371/journal.pone.0044911 (2012)

L.S. Lawson, and T. Huser, “Synthesis and Characterization of a disulfide reporter molecule for enhancing pH-measurements based on surface-enhanced Raman scattering (SERS)”, Anal. Chem. 84(8), 3574–3580 (2012)

I. Yahiatène, S. Doose, T. Huser, and M. Sauer, “Correlation-Matrix Analysis of Two-Color Coincidence Events in Single-Molecule Fluorescence Experiments”, Anal. Chem. 84(6), 2729–2736 (2012)

Z.J. Smith, T. Huser, and S. Wachsmann-Hogiu,”Raman scattering in pathology”, Analytical Cellular Pathology 35(3), 145-163 (2012) – Invited Review Article

B.M. Dale, G.P. McNerney, D.L. Thompson, W. Hubner, K. de los Reyes, F.Y.S. Chuang, T. Huser, and B.K. Chen, “Endosomal HIV-1 particle maturation activates viral membrane fusion following transfer across virological synapses”, Cell Host and Microbe 10, 551-562 (2011)

S. Ly, J. Petrlova, T. Huser, S. Fore, T. Gao, J. Voss, and T.A. Laurence, “Stoichiometry of reconstituted high density lipoproteins in the hydrated state determined by photon antibunching”, Biophys. J. 101(4), 970-975 (2011)

I.W. Schie, J. Wu, T. Weeks, M.A. Zern, J.C. Rutledge, and T. Huser, “Label-free imaging and analysis of the effects of lipolysis products on primary hepatocytes”, J. Biophoton., 4(6), 425-434 (2011)

T. Monson, C. Hollars, C. Orme, and T. Huser, “Improving Nanoparticle Dispersion and Charge Transfer in Cadmium Telluride Tetrapod and Conjugated Polymer Blends”, ACS Applied Materials & Interfaces 3(4), 1077-1082 (2011)

S. Fore, J. Chan, D. Taylor, and T. Huser, “Raman spectroscopy of individual monocytes reveals that single-beam optical trapping of mononuclear cells occurs by their nucleus”, J. Opt. 13(4), 044021-1 - 044021-9 (2011)

B. Dale, W. Hubner, G. McNerney, T. Huser, and B.K. Chen, “Tracking and quantitation of fluorescent HIV during cell-cell transmission”, Methods 53(1), 20 - 26 (2011)

T. Weeks, I. Schie, L.J. den Hartigh, J.C. Rutledge, T. Huser, “Lipid-cell interactions in human monocytes investigated by doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS) microscopy”, J. Biomed. Opt. 16(2), 021117-1 - 021117-5 (2011)

T. Gao, C. Blanchette, W. He, F. Bourguet, S. Ly, F. Katzen, W. Kudlicki, P. Henderson, T. Laurence, T. Huser, and M.A. Coleman, “Characterizing diffusion dynamics of a membrane protein associated with nanolipoproteins using fluorescence correlation spectroscopy”, Protein Science 20(2), 437-447 (2011)

B. Dale, G.P. McNerney, D.L. Thompson, T. Huser, and B.K. Chen, “Visualizing cell-to-cell transfer of HIV using fluorescent clones of HIV and live confocal microscopy”, J. Vis. Exp. 44 (2010) http://www.jove.com/index/details.stp?id=2061, doi: 10.3791/2061

T.J. Weeks, and T.R. Huser, “Differential imaging of biological structures with doubly-resonant coherent anti-Stokes Raman scattering (DR-CARS)”, J. Vis. Exp. 44 (2010) http://www.jove.com/index/details.stp?id=2085, doi: 10.3791/2085

B.S. Ahluwalia, P. McCourt, T. Huser, and O.G. Hellesø, “Optical trapping and propulsion of red blood cells on waveguide surfaces”, Opt. Express 20(18), 21053 - 21061 (2010)

V.C. Cogger, G.P. McNerney, T. Nyunt, L. DeLeve, P. McCourt, B. Smedsrød, D.G. Le Couteur, and T.R. Huser, “Three-dimensional, structured illumination microscopy of liver sinusoidal endothelial cell fenestrations”, J. Struct. Biol. 171, 382 - 388 (2010)

G.P. McNerney, W. Hübner, B.K. Chen, and T. Huser, “Manipulating CD4+ T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1”, J. Biophoton. 3(4), 216 – 223 (2010)

L.J. den Hartigh, J.E. Connolly-Rohrbach, S. Fore, T.R. Huser, J.C. Rutledge, “Fatty acids from Very Low-Density Lipoprotein Lipolysis Products Induce Lipid Droplet Accumulation in Human Monocytes”, J. Immunol., 184(7), 3927 - 3936 (2010)

T. Weeks, I. Schie, S. Wachsmann-Hogiu, and T. Huser, “Signal generation and Raman-resonant imaging by non-degenerate four-wave mixing under tight focusing conditions” J. Biophoton. 3(3), 169 – 175 (2010)

Aktuelle Forschungsthemen

Improving the sensitivity, spatial and temporal resolution of single cell and single molecular processes by label free optical microscopy