201143 Genome Research (V) (WiSe 2002/2003)

The lecture Genome Research gives a broad introduction in genome research in prokaryotes and eukaryotes using selected model organisms. First, the theoretical and methodological basis of structural genomics is covered, in particular basic knowledge in genome organization, genome mapping, genome and EST-sequencing, comparative genome organization, genome and EST annotation. The second part of the lecture focuses on functional genomics. Here, the theoretical and methodological basis of transcriptomics (chip- and array-technology) and proteomics (2d-gel electrophoresis and mass spectroscopy) is presented. Subsequently, approaches of functional genomics are presented that rely on the analysis of mutant phenotypes. Finally, approaches of comparative genomics will be covered that try to transfer knowledge obtained in model organisms to organisms that are of importance e.g. in agriculture. Current genome research projects on prokaryotes, unicellular eukaryotes and higher eukaryotes will be presented in appropriate sections of the lecture. [Lf: n; 50: n; Fs: n