201051 Block: Bioscreening und in-vivo Detektion (Pr+Ü) (SoSe 2006)

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Termin III, Vorbesprechung: 07.06., 13 s.t., W5-135

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Gegenstand des diesjährigen Praktikums ist die Charakterisierung von Signalerkennung, Signalverarbeitung und zellulären Regulationsprozessen unter dem Einfluss verschiedener abiotischer Umweltfaktoren wie Trockenheit, Kälte, Hitze, Starklicht und Salz in Wildtyp- und transgenen Arabidopsis-Pflanzenlinien. Im Praktikum werden folgende Methoden eingesetzt: Einführung in Experimentplanung und -durchführung, Isolierung von Nukleinsäuren aus pflanzlichen Geweben, Untersuchung der Genexpression mittels RT-PCR und Analyse von Promoter-Reportergen-Linien, subzelluläre Lokalisierung von fluoreszenzmarkierten Fusionsproteinen (green fluorescent protein) mittels digitaler Fluoreszenzmikroskopie und konfokaler laserscanningmikroskopie, Einführung in die Literaturrecherche, Anleitung zu wissenschaftlichen Präsentation und Diskussion. Erfolgskontrolle: Seminarvortrag, Abschlusspräsentation, Protokoll

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Subject assignments

Degree programme/academic programme Validity Variant Subdivision Status Semester LP  
Biologie / Diplom (Enrollment until WiSe 02/03) c+f Wahlpflicht HS
Biologie / Lehramt Sekundarstufe II A2+B2 Wahlpflicht HS

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Registered number: 3
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Limited number of participants: 12
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SS2006_201051@ekvv.uni-bielefeld.de
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Last update basic details/teaching staff:
Friday, December 11, 2015 
Last update times:
Friday, December 2, 2005 
Last update rooms:
Friday, December 2, 2005 
Type(s) / SWS (hours per week per semester)
internship (Pr) + exercise (Ü) / 8+2
Department
Faculty of Biology
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ID
677324