206152 Molekulare Physiologie A (S+Pr) (SoSe 2015)
Short comment
- Zentrale Vergabe der Praktikumsplätze für die Spezialmodule
Contents, comment
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In diesem Modul werden molekularbiologische Techniken und konzeptionelle Strategien zur Herstellung und funktionellen Charakterisierung transgener Organismen vermittelt.
Im Praktikum wird eine komplette Klonierungsstrategie (von der Herstellung des chimären Genkonstrukts zur Transformation und der Charakterisierung der transgenen Pflanzen) durchgeführt.
Techniken, die bei diesem Modul vermittelt werden, sind unter anderem:
➢ In silico design einer Klonierstrategie mit Clonemanager® Software
➢ Plasmidisolation, Restriktion und Ligation von Vektoren, Herstellung chemo-kompetenter Bakterien
➢ Transformationen von Escherichia coli und Agrobacterium tumefaciens
➢ Methoden der Pflanzenanzucht
➢ Transformation der Modellpflanze Arabidopsis thaliana
➢ Selektion transgener Pflanzen
➢ Reportergen Assays
➢ Mikroskopie
➢ Kreuzen von Arabidopsis
➢ Untersuchung lichtabhängiger Genexpression in der Nutzpflanze Hordeum vulgare (Gerste) mittels Immunoblotanalysen
➢ Chemilumineszenzdetektion und Quantifizierung von Proteinen mittels der Aida® SoftwareFerner werden Hinweise und Tipps zu Präsentationstechniken gegeben. Gelegenheit zum Üben bietet das Abschlusskolloquium.
Teaching staff
Dates ( Calendar view )
| Frequency | Weekday | Time | Format / Place | Period |
|---|
Subject assignments
| Module | Course | Requirements | |
|---|---|---|---|
| 20-SM52 Spezialmodul Molekulare Physiologie | Praktikum im SM52 | Study requirement
Ungraded examination |
Student information |
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- SS2015_206152@ekvv.uni-bielefeld.de
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- Notes:
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- Last update basic details/teaching staff:
- Friday, December 11, 2015
- Last update times:
- Monday, January 26, 2015
- Last update rooms:
- Monday, January 26, 2015
- Type(s) / SWS (hours per week per semester)
- seminar (S) + internship (Pr) / 1+5
- Department
- Faculty of Biology
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