202107 Spezialmodul "Biotechnologische und molekularbiologische Methoden in Pflanzen" (Ü+Pr) (WiSe 2021/2022)

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Im Spezialmodul werden Beispiele biotechnologischer Verbesserungen von Pflanzen hinsichtlich der Toleranz gegenüber Überflutungsstress und anderen abiotische Stressformen aufgezeigt. Hierzu zählen die Klonierung und Modifikation von spezifischen molekularen Signalkomponenten unter Stress zu deren funktionellen Charakterisierung. Zudem führen wir Analysen hinsichtlich der phänotypischen, transkriptionellen und metabolischen Anpassungen an Stress durch. Folgende Methoden können angewendet werden: Genaktivitätsstudien mittels RNA-Extraktion, cDNA-Synthese und RT-qPCR-basierter Quantifizierung, Genaktivitätsmuster ausgewählter Stressgene mittels des GUS-Systems, Bestimmung von Proteinmengen von Signalkomponenten unter Stress, Transkriptionsfaktoraktivitäten im Protoplasten-System (Transaktivierungsassays mit Lumineszenzbasierten Reportergenen), Protein-Protein-Interaktionsstudien im heterologen System Hefe, Toleranzassays mit Arabidopsis-Mutanten, Datenanalyse und Vorbereitung eines Fachvortrags.

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20-SM16 Biotechnologie der Pflanzen Biotechnologie der Pflanzen Study requirement
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Limited number of participants: 12
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WS2021_202107@ekvv.uni-bielefeld.de
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Last update basic details/teaching staff:
Wednesday, January 19, 2022 
Last update times:
Monday, July 19, 2021 
Last update rooms:
Monday, July 19, 2021 
Type(s) / SWS (hours per week per semester)
Ü+Pr / 3+5
Department
Faculty of Biology
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290391881